Reticulum Stain: A Guide to Histological Staining of Reticular Fibers

Introduction:

In the field of histology, Reticulum Stain, also known as ACMBS-35, is a specialized product designed for staining reticular fibers within tissue samples. This blog post will provide an overview of the product, its main components, sample requirements, test procedure, result interpretation, and important precautions to ensure effective and accurate staining results.

Product Details:

• Specifications: Available in 7x10mL and 7x100mL boxes
• Intended use: Primarily used for histological staining of reticular fibers

Principle of Staining:

Reticulum Stain utilizes the Ammoniacal silver solution, which is adsorbed by tissue and reacts with tissue proteins through formaldehyde reduction. This process leads to the deposition of black metallic silver on the surface and inside the tissue, specifically highlighting the reticular fibers. The procedure also involves toning with gold chloride and washing with sodium thiosulfate solution to clearly display the reticulum within the tissue.

Main Components of Reticulum Stain:

The main ingredients of Reticulum Stain are as follows:

• The main ingredients of Reticulum Stain are as follows:
• Potassium permanganate arrangement: Contains Potassium permanganate, utilized for oxidation.
• Oxalic corrosive arrangement: Comprises of Oxalic corrosive and goes about as a fading specialist.
• Ferric ammonium sulfate arrangement: Contains Ferric ammonium sulfate, an essential piece of the staining system.
• Ammoniacal silver arrangement: Contains Silver nitrate and Smelling salts, fundamental for the silver affidavit.
• Formaldehyde solution: Utilized for formaldehyde reduction in the tissue.
• Gold chloride solution: Used for toning the stained tissue.
• Sodium thiosulphate solution: Utilized to wash unreduced silver.

Sample Requirements:

For successful staining, the tissue section should have a thickness of about 5μm and must be fully fixed.

Test Procedure:

• Dewax the tissue slices to the water.
• Oxidize the substance using a solution of Potassium permanganate for a duration of three minutes, followed by rinsing with running tap water.
• To achieve a colorless result, immerse the tissue in an Oxalic acid solution for a duration of 1 to 2 minutes. Afterward, ensure to rinse it thoroughly with water.
• Soak in the Ferric ammonium sulfate solution for a duration of 3 minutes, followed by a 10-second rinse in deionized water.
• Apply Ammoniacal silver solution for 5 minutes, ensuring immediate tight capping of the bottle to prevent ammonia volatilization.
• Reduce with Formaldehyde solution for 30 seconds, then wash with running tap water.
• Tone with Gold chloride solution for 1 minute, and if necessary, control under a microscope before washing with running tap water.
•  Apply Sodium thiosulphate solution for 2 minutes, then wash with running tap water.
• Conventional dehydration and transparent process, followed by sealing with neutral resin.

Result Interpretation:

• Reticular fibers: Appear black or blue-black after staining.
• Collagen fibers: Exhibit a pink coloration.

Precautions:

• Store Ammoniacal silver solution away from light and at a low temperature.
• Pre-soak and rinse all containers used in the procedure with a cleaning solution.
• This staining reagent should be handled by professionals, and instructions must be carefully read and followed. Adhere to personal hygiene protection.
• Dispose of waste according to the hospital or environmental protection department requirements.
• Check the production date, batch number, and expiration date on the outer packaging.

Reference:

• The information provided here is based on “Practical Pathology Special Staining and Histochemistry Technology” by Ling Qibo, published by Guangdong Higher Education Press.

Conclusion:

Reticulum Stain, with its precise staining process and specific result interpretation, plays a crucial role in histological studies focusing on reticular fibers. By following the recommended test procedure and precautions, histologists can achieve accurate and reliable staining results, enabling them to advance their research and understanding of tissue structures.

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