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In histological analysis, the Giemsa staining method is a widely used technique for visualizing adrenal chromaffin cells. This blog provides a detailed overview of the Giemsa staining procedure, its main components, sample requirements, and result interpretation. It also highlights important precautions to ensure accurate results and safety during the process.
Specifications: 25mL + 500mL (Box)
Intended Use: Used for staining of histological adrenal chromaffin cells
Store Temperature: 5℃～30℃
Principle of Giemsa Staining
The Giemsa staining method involves staining tissue fixed with a chromium salt fixative using Giemsa dye. This results in the chromaffin cell granules being stained green-yellow. The staining helps researchers and medical professionals observe the cytoplasmic granules, nucleus, and connective tissue in the sample.
Main Components and Reagent Composition
The Giemsa staining kit (ACMBS-64) contains two main solutions:
- Giemsa A Solution: Contains Giemsa dye.
- Giemsa B Solution: Contains phosphate.
Before staining, fresh tissue must undergo specific preparation:
- The tissue is immediately placed in Regaud’s solution for 2 to 4 days.
- The solution is changed daily.
- The tissue is then fixed with a 3% potassium dichromate aqueous solution for 1 day.
- After fixing, the tissue is rinsed with running water for 24 hours and dehydrated conventionally.
- The tissue section must be fully fixed before proceeding with the staining process.
Follow these steps for the Giemsa staining procedure:
- Dewax the tissue slices and wash them once with distilled water for approximately 30 minutes.
- Dip the tissue slices into Giemsa diluent (A Solution: B Solution = 1:20) and let them stain for 18 to 24 hours.
- After staining, wash the tissue slices slightly with distilled water and dry them with filter paper.
- Differentiate the tissue slices for a few seconds using n-butanol (purchased separately) and then dry them with filter paper.
- Make the tissue slices transparent using xylene and seal them with neutral resin.
The Giemsa staining results are interpreted as follows:
- Chromaffin Cell Cytoplasmic Granules: Stained green-yellow.
- Nucleus: Stained blue.
- Connective Tissue: Stained light red.
To ensure safety and accuracy during the staining process, adhere to these precautions:
- Prepare Regaud’s fixative by mixing 3% potassium dichromate (80ml) and concentrated formaldehyde solution (20ml) before use. Use it within 24 hours of preparation.
- This reagent should only be used by professionals who can interpret the results. Read the instruction manual carefully and practice personal hygiene protection during use.
- Dispose of waste according to hospital or environmental protection department requirements after use.
- Check the production date, batch number, and expiration date on the outer packaging.
- Chinese Medical Association. Clinical Technical Operating Specifications – Pathology Volumes. People’s Military Medical Publishing House.
- Ling Qibo. Practical Pathology Special Staining and Histochemistry Technology. Guangdong Higher Education Press.
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