Adrenal Chromaffin Cell Stain (Giemsa Staining Method)

Introduction

In histological analysis, the Giemsa staining method is a widely used technique for visualizing adrenal chromaffin cells. This blog provides a detailed overview of the Giemsa staining procedure, its main components, sample requirements, and result interpretation. It also highlights important precautions to ensure accurate results and safety during the process.

Product Details

Specifications: 25mL + 500mL (Box)

Intended Use: Used for staining of histological adrenal chromaffin cells

Store Temperature: 5℃～30℃

Principle of Giemsa Staining

The Giemsa staining method involves staining tissue fixed with a chromium salt fixative using Giemsa dye. This results in the chromaffin cell granules being stained green-yellow. The staining helps researchers and medical professionals observe the cytoplasmic granules, nucleus, and connective tissue in the sample.

Main Components and Reagent Composition

The Giemsa staining kit (ACMBS-64) contains two main solutions:

• Giemsa A Solution: Contains Giemsa dye.
• Giemsa B Solution: Contains phosphate.

Sample Requirement

Before staining, fresh tissue must undergo specific preparation:

• The tissue is immediately placed in Regaud’s solution for 2 to 4 days.
• The solution is changed daily.
• The tissue is then fixed with a 3% potassium dichromate aqueous solution for 1 day.
• After fixing, the tissue is rinsed with running water for 24 hours and dehydrated conventionally.
• The tissue section must be fully fixed before proceeding with the staining process.

Test Procedure

Follow these steps for the Giemsa staining procedure:

• Dewax the tissue slices and wash them once with distilled water for approximately 30 minutes.
• Dip the tissue slices into Giemsa diluent (A Solution: B Solution = 1:20) and let them stain for 18 to 24 hours.
• After staining, wash the tissue slices slightly with distilled water and dry them with filter paper.
• Differentiate the tissue slices for a few seconds using n-butanol (purchased separately) and then dry them with filter paper.
• Make the tissue slices transparent using xylene and seal them with neutral resin.

Result Interpretation

The Giemsa staining results are interpreted as follows:

• Chromaffin Cell Cytoplasmic Granules: Stained green-yellow.
• Nucleus: Stained blue.
• Connective Tissue: Stained light red.

Precautions

To ensure safety and accuracy during the staining process, adhere to these precautions:

• Prepare Regaud’s fixative by mixing 3% potassium dichromate (80ml) and concentrated formaldehyde solution (20ml) before use. Use it within 24 hours of preparation.
• This reagent should only be used by professionals who can interpret the results. Read the instruction manual carefully and practice personal hygiene protection during use.
• Dispose of waste according to hospital or environmental protection department requirements after use.
• Check the production date, batch number, and expiration date on the outer packaging.

References

• Chinese Medical Association. Clinical Technical Operating Specifications – Pathology Volumes. People’s Military Medical Publishing House.
• Ling Qibo. Practical Pathology Special Staining and Histochemistry Technology. Guangdong Higher Education Press.

Company Profile: Click Here   

Related Product: Click Here.

BASCO: Click Here.